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Catalog No: OKEH02837
Size:96T
Price: $525.00
SKU
OKEH02837
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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MT1A ELISA Kit (Human) : 96 Wells (OKEH02837)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MT1A ELISA Kit (Human) (OKEH02837) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MT1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MT1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MT1A captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MT1A Microplate96 Wells (12 x 8 Well strips)
MT1A Lyophilized Standard2
100X Biotinylated MT1A Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.79 ng/mL
SpecificityNatural and recombinant Human Metallothionein-1A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMT1A
Gene Full Namemetallothionein 1A
Alias Symbolsmetallothionein 1A (functional);metallothionein 1S;metallothionein-1A;metallothionein-IA;MT1;MT-1A;MT1S;MTC;MT-IA.
NCBI Gene Id4489
Protein NameP04731
Description of TargetMetallothionein-1A
Uniprot IDhttps://www.uniprot.org/uniprot/P04731
Protein Accession #NP_005937.2
Nucleotide Accession #NM_005946.2
Protein Size (# AA)61
Molecular Weight6 kDa
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