MSTO1 ELISA Kit (Mouse) (OKCD01100)

Catalog No: OKCD01100 (Formerly GWB-KBBIU7)

Size:96WELLS

Mouse Msto1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	90-103%	87-94%	82-102%	79-94%
  EDTA plasma(n=5)	80-92%	85-94%	88-96%	97-104%
  heparin plasma(n=5)	95-102%	93-105%	84-97%	93-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Misato Homolog 1 (MSTO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Misato Homolog 1 (MSTO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Misato Homolog 1 (MSTO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Misato Homolog 1 (MSTO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-94	81
  EDTA plasma(n=5)	83-93	89
  heparin plasma(n=5)	89-104	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Misato Homolog 1 (MSTO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Misato Homolog 1 (MSTO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Msto1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Msto1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated Msto1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Misato Homolog 1 (MSTO1). No significant cross-reactivity or interference between Misato Homolog 1 (MSTO1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Msto1
• Gene Full Name: misato 1, mitochondrial distribution and morphology regulator
• Alias Symbols: BC008103;misato homolog 1;mst;protein misato homolog 1.
• NCBI Gene Id: 229524
• Protein Name: Protein misato homolog 1
• Description of Target: Involved in the regulation of mitochondrial distribution and morphology. Required for mitochondrial fusion and mitochondrial network formation.
• Uniprot ID: Q2YDW2
• Protein Accession #: NP_659147
• Nucleotide Accession #: NM_144898.2
• Protein Size (# AA): 556