Catalog No: OKCD02735
Size:96WELLS
Price: $750.00
SKU
OKCD02735
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Datasheets/ManualsPrintable datasheet for MSTN ELISA Kit (Pig) (OKCD02735)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-92%81-99%79-99%82-104%
EDTA Plasma (n=5)84-92%80-93%96-103%95-105%
Heparin Plasma (n=5)80-103%90-97%85-101%85-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myostatin (MSTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myostatin (MSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myostatin (MSTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myostatin (MSTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range125-8,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)93-10498
EDTA Plasma (n=5)84-9389
Heparin Plasma(n=5)89-9692
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myostatin (MSTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myostatin (MSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MSTN Microplate96 Wells (12 x 8 Well strips)
MSTN Lyophilized Standard2
100X Biotinylated MSTN Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity53 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myostatin (MSTN). No significant cross-reactivity or interference between Myostatin (MSTN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMSTN
Alias SymbolsMyostatin.
Protein NameGrowth/differentiation factor 8
Description of TargetActs specifically as a negative regulator of skeletal muscle growth.
Uniprot IDO18831
Protein Accession #NP_999600.2
Nucleotide Accession #NM_214435.2
Protein Size (# AA)375
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