MST1R Antibody (Phospho-Ser1394) (OAAF00172)

Catalog No: OAAF00172 (Formerly GWB-ASB370)

Size:100 ug

Western blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml 2', using CD136/RON (Phospho-Ser1394) Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid (without Mg²⁺ and Ca²⁺) (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
  Modification Sites: H:S1394, M:S1372
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human CD136/RON around the phosphorylation site of Ser1394.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Concentration: 1 mg/ml
• Specificity: CD136/RON (Phospho-Ser1394) antibody detects endogenous levels of CD136/RON only when phosphorylated at serine 1394.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1000
  ELISA: 1:40000
• Reference: Lily Lai, J. Immunol., Apr 1998; 160: 3861.
  Warren Casey, Physiol Genomics, Feb 2002; 8: 115.
  BENJAMIN P. C. CHEN, Physiol Genomics, Oct 2001; 7: 55.
  Christa E. Muller-Sieburg, Blood, Jun 2004; 103: 4111 - 4118.

Target Info

• Gene Symbol: MST1R
• Gene Full Name: macrophage stimulating 1 receptor
• Alias Symbols: CD136;CDw136;c-met-related tyrosine kinase;macrophage-stimulating protein receptor;MSP receptor;NPCA3;p185-Ron;Protein-tyrosine kinase 8;PTK8;PTK8 protein tyrosine kinase 8;RON;S13 avian erythroblastosis oncogene homolog;S13 erythroblastosis oncogene homolog;SEA.
• NCBI Gene Id: 4486
• Protein Name: Macrophage-stimulating protein receptor
• Description of Target: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
• Uniprot ID: Q04912
• Protein Accession #: NP_001231866.1
• Molecular Weight: 152 kDa