MST1 ELISA Kit (Rat) (OKCD00267)

Catalog No: OKCD00267 (Formerly GWB-KBBVI6)

Size:96WELLS

Rat Mst1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	91-105%	81-97%	84-101%	83-91%
  EDTA plasma(n=5)	85-102%	87-95%	78-96%	80-98%
  heparin plasma(n=5)	84-93%	80-92%	79-101%	78-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Stimulating Protein (MSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Stimulating Protein (MSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Stimulating Protein (MSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Macrophage Stimulating Protein (MSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	81-91	85
  EDTA plasma(n=5)	85-93	89
  heparin plasma(n=5)	78-92	81

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Stimulating Protein (MSP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Stimulating Protein (MSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mst1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mst1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Mst1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Macrophage Stimulating Protein (MSP). No significant cross-reactivity or interference between Macrophage Stimulating Protein (MSP) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Mst1
• Gene Full Name: macrophage stimulating 1
• Alias Symbols: D8h3f15s2;E2F transcription factor 2;E2F2;hepatocyte growth factor-like protein;Macrophage stimulating 1 (hepatocyte growth factor-like).
• NCBI Gene Id: 24566
• Protein Name: Macrophage stimulating 1 (Hepatocyte growth factor-like)
• Description of Target: Macrophage stimulating 1 (Hepatocyte growth factor-like)
• Uniprot ID: P70521
• Protein Accession #: NP_077328
• Nucleotide Accession #: NM_024352.1
• Protein Size (# AA): 716