MSLN ELISA Kit (Mouse) (OKCD09073)

Catalog No: OKCD09073

Size:96WELLS

MSLN ELISA Kit (Mouse) (OKCD09073) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesothelin (MSLN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mesothelin (MSLN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mesothelin (MSLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mesothelin (MSLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mesothelin (MSLN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mesothelin (MSLN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MSLN Microplate: 96 Wells (12 x 8 Well strips)
  • MSLN Lyophilized Standard: 2
  • 100X MSLN HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: Msln
• Gene Full Name: mesothelin
• Alias Symbols: megakaryocyte potentiating factor;mesothelin;MP;MPF;pre-pro-megakaryocyte-potentiating factor.
• NCBI Gene Id: 56047
• Protein Name: Mesothelin
• Description of Target: Membrane-anchored forms may play a role in cellular adhesion.
• Uniprot ID: Q61468
• Protein Accession #: NP_061345.1
• Nucleotide Accession #: NM_018857.1