MSH6 Antibody (OASL01202)

Catalog No: OASL01202

Size:50ul

Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4C, overnight) . 2, Tris-EDTA, pH8.0 was used for antigen retrieval.

Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4C, overnight) . 2, Tris-EDTA, pH8.0 was used for antigen retrieval.

Product Info

• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Monoclonal
• Isotype: IgG2a, Kappa
• Host: Mouse
• Application: IHC-P
• Additional Information: Background: mutS homolog 6(MSH6) Homo sapiens this gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.
  Function: disease: defects in MSH6 are a cause of susceptibility to endometrial cancer [MIM: 608089]. Disease: defects in MSH6 are the cause of hereditary non-polyposis colorectal cancer type 5 (HNPCC5) [MIM: 600678]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Cancers in HNPCC originate within benign neoplastic polyps.
  Location: nuclear
  Expression: Epithelium, Placenta, Pooled, Testis.
• Reconstitution and Storage: Store at -20C for one year. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human MutS Protein Homolog 6(MSH6)
• Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Concentration: 1 mg/ml
• Specificity: This antibody detects endogenous levels of human MutS Protein Homolog 6(MSH6)
• Application Info: IHC-P 1:200

Target Info

• Gene Symbol: MSH6
• Alias Symbols: GTBP, HSAP, p160, GTMBP, HNPCC5, MMRCS3
• NCBI Gene Id: 2956
• Protein Name: DNA mismatch repair protein Msh6 (hMSH6) (G/T mismatch-binding protein) (GTBP) (GTMBP) (MutS-alpha 160 kDa subunit) (p160)
• Uniprot ID: P52701