MSH2 Antibody (OAAB21482)

Catalog No: OAAB21482

Size:100ul

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB
• Additional Information: Function: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Application Info: WB: 1:1000
• Reference: Fishel R.,et al.Cell 75:1027-1038(1993).
  Fishel R.,et al.Cell 77:167-167(1994).
  Leach F.S.,et al.Cell 75:1215-1225(1993).
  Kolodner R.D.,et al.Genomics 24:516-526(1994).
  Wijnen J.,et al.Am. J. Hum. Genet. 56:1060-1066(1995).
  

Target Info

• Gene Symbol: MSH2
• Gene Full Name: mutS homolog 2
• Alias Symbols: FCC1, COCA1, HNPCC, LCFS2, hMSH2, HNPCC1, MMRCS2
• NCBI Gene Id: 4436
• Description of Target: This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P43246
• Protein Accession #: NP_000242.1
• Nucleotide Accession #: NM_000251.2
• Molecular Weight: 105 kDa