MRPS5 Antibody (OAGA00267)

Catalog No: OAGA00267

Size:100 ul

Sample(30 ug whole cell lysate)
A:A431
B:H1299
C:HeLa S3
D:Hep G2
10% SDS PAGE
OAGA00267 diluted at 1:1000

MRPS5 antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
MRPS5 antibody (OAGA00267) dilution: 1:500.

Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5(OAGA00267) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q. [provided by RefSeq]
  Positive Controls: A431, H1299, HeLaS3, HepG2
  Cellular Localization: Mitochondrion
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 430 of MRPS5 (Uniprot ID#P82675)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: MRPS5
• Gene Full Name: 28S ribosomal protein S5, mitochondrial
• Alias Symbols: S5mt, MRP-S5
• NCBI Gene Id: 64969
• Protein Name: 28S ribosomal protein S5, mitochondrial
• Uniprot ID: P82675
• Protein Accession #: NP_114108.1
• Molecular Weight: 48 kDa