Catalog No: OKCD02732
Size:96WELLS
Price: $625.00
SKU
OKCD02732
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Datasheets/ManualsPrintable datasheet for MRC2 ELISA Kit (Mouse) (OKCD02732)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-105%96-104%87-94%80-99%
EDTA Plasma (n=5)83-101%88-102%94-102%95-103%
Heparin Plasma (n=5)78-90%91-98%95-103%79-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 2 (MRC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Receptor C Type 2 (MRC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 2 (MRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mannose Receptor C Type 2 (MRC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)86-9589
EDTA Plasma (n=5)97-105102
Heparin Plasma(n=5)78-9584
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Receptor C Type 2 (MRC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Receptor C Type 2 (MRC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-MRC2 Microplate96 Wells (12 x 8 Well strips)
MRC2 Lyophilized Standard2
100X Biotinylated MRC2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity6.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mannose Receptor C Type 2 (MRC2). No significant cross-reactivity or interference between Mannose Receptor C Type 2 (MRC2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolMrc2
Gene Full Namemannose receptor, C type 2
Alias SymbolsC-type mannose receptor 2;Endo1;Endo180;lectin lambda;macrophage mannose receptor 2;mKIAA0709;novel lectin;u;uPARAP.
NCBI Gene Id17534
Protein NameC-type mannose receptor 2
Description of TargetMay play a role as endocytotic lectin receptor displaying calcium-dependent lectin activity. Internalizes glycosylated ligands from the extracellular space for release in an endosomal compartment via clathrin-mediated endocytosis. May be involved in plasminogen activation system controlling the extracellular level of PLAUR/PLAU, and thus may regulate protease activity at the cell surface. May contribute to cellular uptake, remodeling and degradation of extracellular collagen matrices. May participate in remodeling of extracellular matrix cooperating with the matrix metalloproteinases (MMPs).
Uniprot IDQ64449
Protein Accession #NP_032652.3
Nucleotide Accession #NM_008626.3
Protein Size (# AA)1479
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