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Mrc1 Antibody (OASA05048)

25ug
$100.00
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Gene Symbol:
Mrc1
NCBI Gene Id:
17533
Protein Name:
Macrophage mannose receptor 1
Swissprot Id:
Q61830
Protein Accession #:
NP_032651.2
NM_005625
Alias Symbols:
RAT ANTI MOUSE CD206; CD206; MANNOSE RECEPTOR C TYPE 1
Replacement Item:
This antibody may replace item sc-34577 from Santa Cruz Biotechnology.
Description of Target:
The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins. Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands. Clone MR5D3 has also been shown to work in Western Blot.
Host:
Rat
Clonality:
Monoclonal
Purification:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Application:
FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Mrc1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Mrc1.
Immunogen:
Chimaeric CRD4-7-Fc protein.
Product Format:
Liquid PBS with 0.09% sodium azide
Reconstitution and Storage:
Store at 4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate, microcentrifuge before use.
Shelf Life: 18 months from date of dispatch
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-Mrc1 antibody - OASA05048
Specificity:
Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a 175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells.
Clone:
MR5D3
Conjugation:
Purified
Isotype:
IgG2a
Application Info:
FC: 1/10-1/20. Use 10 uL of the suggested working dilution to label 106 cells in 100 uL. CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Additional Information:
Fusion Partners: Spleen cells from immunized Fischer rats were fused with cells of the Y3 myeloma cell line.

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