Mrc1 Antibody (OASA05048)
|Predicted Species Reactivity||Mouse|
|Product Format||LiquidPBS with 0.09% sodium azide|
|Application||FC, IHC-F, IP, IF|
|Additional Information||Fusion Partners: Spleen cells from immunized Fischer rats were fused with cells of the Y3 myeloma cell line.|
|Reconstitution and Storage||Store at 4C or at -20C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate, microcentrifuge before use.|
|Replacement Item||This antibody may replace item sc-34577 from Santa Cruz Biotechnology.|
|Immunogen||Chimaeric CRD4-7-Fc protein.|
|Purification||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant|
|Datasheets/Manuals||Printable datasheet for anti-Mrc1 antibody - OASA05048|
|Specificity||Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a 175 kDa type 1 membrane glycoprotein that is also known as CD206.|
|Application Info||FC: 1/10-1/20 (Use 10 uL of the suggested working dilution to label 106 cells in 100 uL.) CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
Optimal dilutions should be determined by the end user.
|Alias Symbols||RAT ANTI MOUSE CD206, CD206, MANNOSE RECEPTOR C TYPE 1|
|NCBI Gene Id||17533|
|Protein Name||Macrophage mannose receptor 1|
|Description of Target||CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells
The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins.
Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands. Clone MR5D3 has also been shown to work in Western Blot.
|Protein Accession #||NP_032651.2|
|Nucleotide Accession #||NM_005625|
|Molecular Weight||175 kDa|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express Mrc1.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express Mrc1.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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