Mrc1 Antibody - FITC Conjugated (OASA05053)

Catalog No: OASA05053 (Formerly GWB-Q01451)

Size:50UG

Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm (BUF09)

Staining of mouse peritoneal macrophage with Rat anti Mouse CD206: Alexa Fluor 488

Staining of mouse peritoneal macrophage with Rat antti Mouse CD206: Alexa Fluor 647

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: MR5D3
• Isotype: IgG2a
• Host: Rat
• Conjugation: FITC
• Application: Flow cytometry
• Additional Information: Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line.
  Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  1% - Bovine Serum Albumin
  Approx Protein Conc: IgG concentration 0.1 mg/ml
  Buffer Solutions: Phosphate buffered saline
• Reconstitution and Storage: -20°C
• Immunogen: Chimaeric CRD4-7-Fc protein
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: 0.1 mg/ml
• Specificity: Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a 175kD type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells (Zamze et al. 2002).
  The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005).
  Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).
  
• Application Info: CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
  Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using Mouse SeroBlock FcR ( OOSA09564).
  
• Application Data: Application #1: Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm (BUF09)
  Application #2: Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488
  Application #3: Staining of mouse peritoneal macrophages with Rat antti Mouse CD206: Alexa Fluor 647
• Reference: 1. Martinez-Pomares, L. et al. (2003) Analysis of mannose receptor regulation by IL-4, IL-10 and proteolytic processing using novel monoclonal antibodies. J. Leuk. Biol. 73: 604-613.
  2. Zamze, S. et al. (2002) Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. J. Biol. Chem. 277: 41613-41623.
  3. Hassan, M.F. et al. (2006) The Schistosoma mansoni hepatic egg granuloma provides a favorable microenvironment for sustained growth of Leishmania donovani. Am J Pathol. 169: 943-53.
  

Target Info

• Gene Symbol: Mrc1
• Alias Symbols: M, MR, CD206, AW259686
• NCBI Gene Id: 17533
• Protein Name: Macrophage mannose receptor 1
• Description of Target: RAT ANTI MOUSE CD206:FITC
  
• Uniprot ID: Q61830
• Protein Accession #: NP_032651.2
• Protein Size (# AA): 1456