MR1 ELISA Kit (Rat) (OKEH04390)

Catalog No: OKEH04390

Size:96T

Rat MR1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Mr1 ELISA Kit (Rat) (OKEH04390) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mr1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Mr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mr1 captured in the well.
• ELISA Kit Range: 78-5000pg/ml
• ELISA Kit Component:
  • Anti-Mr1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Mr1 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Mr1 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39.5 pg/mL
• Specificity: Natural and recombinant Rat Major histocompatibility complex class I-related gene protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mr1
• Gene Full Name: major histocompatibility complex, class I-related
• Alias Symbols: histocompatibility-2 complex class 1-like sequence;Hlals;major histocompatibility complex class I-related gene protein;MHC class I-like sequence;MHC class I-related gene protein.
• NCBI Gene Id: 25119
• Protein Name: O19477
• Description of Target: Major histocompatibility complex class I-related gene protein
• Uniprot ID: https://www.uniprot.org/uniprot/O19477
• Protein Accession #: NP_001094105.1
• Nucleotide Accession #: NM_001100635.1
• Protein Size (# AA): 343
• Molecular Weight: 40 KDa