MPZ ELISA Kit (Human) (OKDD00403)

Catalog No: OKDD00403 (Formerly GWB-KBBTI5)

Size:96 Wells

Human MPZ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology MPZ ELISA Kit (Human) (OKDD00403) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MPZ has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MPZ is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MPZ captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MPZ were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MPZ were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-MPZ Microplate: 96 Wells (12 x 8 Well Strips)
  • MPZ Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X MPZ Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: < 0.052 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of MPZ. No significant cross-reactivity or interference between MPZ and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MPZ
• Gene Full Name: myelin protein zero
• Alias Symbols: Charcot-Marie-Tooth neuropathy 1B;CHM;CHN2;CMT1;CMT1B;CMT2I;CMT2J;CMT4E;CMTDI3;CMTDID;DSS;HMSNIB;MPP;myelin peripheral protein;myelin protein P0;Myelin protein zero;P0.
• NCBI Gene Id: 4359
• Protein Name: Myelin protein P0
• Description of Target: Is an adhesion molecule necessary for normal myelination in the peripheral nervous system. It mediates adhesion between adjacent myelin wraps and ultimately drives myelin compaction.
• Uniprot ID: P25189
• Protein Accession #: NP_000521.2
• Nucleotide Accession #: NM_000530.7
• Protein Size (# AA): 248
• Molecular Weight: 28 kDa