MPST ELISA Kit (Human) (OKCD08208)

Catalog No: OKCD08208

Size:96WELLS

MPST ELISA Kit (Human) (OKCD08208) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Mercaptopyruvate Sulfurtransferase (MST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Mercaptopyruvate Sulfurtransferase (MST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Mercaptopyruvate Sulfurtransferase (MST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 3-Mercaptopyruvate Sulfurtransferase (MST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-Mercaptopyruvate Sulfurtransferase (MST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-Mercaptopyruvate Sulfurtransferase (MST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MPST Microplate: 96 Wells (12 x 8 Well strips)
  • MPST Lyophilized Standard: 2
  • 100X MPST HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.063ng/mL

Target Info

• Gene Symbol: MPST
• Gene Full Name: mercaptopyruvate sulfurtransferase
• Alias Symbols: 3-mercaptopyruvate sulfurtransferase;human liver rhodanese;MST;testicular tissue protein Li 200;tRNA thiouridin modification protein 1;TST2;TUM1.
• NCBI Gene Id: 4357
• Protein Name: 3-mercaptopyruvate sulfurtransferase
• Description of Target: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
• Uniprot ID: P25325
• Protein Accession #: NP_001013454.1
• Nucleotide Accession #: NM_001013436.2