MPP2 ELISA Kit (Rat) (OKCD04446)

Catalog No: OKCD04446

Size:96WELLS

Rat MPP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	89-97%	92-99%	78-89%	78-90%
  EDTA Plasma (n=5)	83-104%	85-94%	88-102%	86-95%
  Heparin Plasma (n=5)	80-93%	89-96%	79-91%	78-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Protein, Palmitoylated 2 (MPP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Membrane Protein, Palmitoylated 2 (MPP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Protein, Palmitoylated 2 (MPP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Membrane Protein, Palmitoylated 2 (MPP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40U/L
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	97-104	101
  EDTA Plasma (n=5)	81-98	85
  Heparin Plasma (n=5)	90-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Membrane Protein, Palmitoylated 2 (MPP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Membrane Protein, Palmitoylated 2 (MPP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Ttr Microplate: 96 Wells (12 x 8 Well strips)
  • Ttr Lyophilized Standard: 2
  • 100X Ttr HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: 0.24 U/L
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MPP2
• Alias Symbols: Dlg2
• NCBI Gene Id: 85275
• Protein Name: MAGUK p55 subfamily member 2
• Description of Target: a protein related to CASK that may play a role in synaptic vesicle exocytosis and synaptic junctions [RGD, Feb 2006]
• Uniprot ID: D3ZAA9
• Protein Accession #: NP_445965.1
• Nucleotide Accession #: NM_053513.1