MPL ELISA Kit (Human) (OKCD07412)

Catalog No: OKCD07412

Size:96WELLS

MPL ELISA Kit (Human) (OKCD07412) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombopoietin Receptor (TPOR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombopoietin Receptor (TPOR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombopoietin Receptor (TPOR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thrombopoietin Receptor (TPOR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombopoietin Receptor (TPOR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombopoietin Receptor (TPOR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • MPL Microplate: 96 Wells (12 x 8 Well strips)
  • MPL Lyophilized Standard: 2
  • 100X MPL HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.113ng/mL
• Publications:

  Rebound Thrombocytosis after Induction Chemotherapy is a Strong Biomarker for Favorable Outcome in AML Patients. Hemasphere. 3, e180 (2019). 31723819

• Description:
  View product citations for TPOR ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: MPL
• Gene Full Name: MPL proto-oncogene, thrombopoietin receptor
• Alias Symbols: CD110;C-MPL;MPLV;myeloproliferative leukemia protein;myeloproliferative leukemia virus oncogene;proto-oncogene c-Mpl;THCYT2;THPOR;thrombopoietin receptor;TPOR;TPO-R.
• NCBI Gene Id: 4352
• Protein Name: Thrombopoietin receptor
• Description of Target: Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.
• Uniprot ID: P40238
• Protein Accession #: NP_005364.1
• Nucleotide Accession #: NM_005373.2