MPI ELISA Kit (Human) (OKCD00121)

Catalog No: OKCD00121 (Formerly GWB-KBBPQ6)

Size:96WELLS

Human MPI ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	81-90%	89-103%	99-105%	92-105%
  EDTA plasma(n=5)	79-102%	79-90%	89-102%	92-105%
  heparin plasma(n=5)	89-96%	78-93%	87-96%	80-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Phosphate Isomerase (MPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Phosphate Isomerase (MPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Phosphate Isomerase (MPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mannose Phosphate Isomerase (MPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	93-105	99
  EDTA plasma(n=5)	85-101	90
  heparin plasma(n=5)	94-103	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Phosphate Isomerase (MPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Phosphate Isomerase (MPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MPI Microplate: 96 Wells (12 x 8 Well Strips)
  • MPI Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated MPI Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mannose Phosphate Isomerase (MPI). No significant cross-reactivity or interference between Mannose Phosphate Isomerase (MPI) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MPI
• Gene Full Name: mannose phosphate isomerase
• Alias Symbols: CDG1B;mannose-6-phosphate isomerase;phosphohexomutase;Phosphomannose isomerase;phosphomannose isomerase 1;PMI;PMI1.
• NCBI Gene Id: 4351
• Protein Name: Mannose-6-phosphate isomerase
• Description of Target: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
• Uniprot ID: P34949
• Protein Accession #: NP_001276085
• Nucleotide Accession #: NM_001289156.1
• Protein Size (# AA): 423