MPG ELISA Kit (Human) (OKCD00542)

Catalog No: OKCD00542 (Formerly GWB-KBBBG3)

Size:96WELLS

Human MPG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Methylpurine DNA Glycosylase (MPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Methylpurine DNA Glycosylase (MPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Methylpurine DNA Glycosylase (MPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-Methylpurine DNA Glycosylase (MPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Methylpurine DNA Glycosylase (MPG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Methylpurine DNA Glycosylase (MPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MPG Microplate: 96 Wells (12 x 8 Well Strips)
  • MPG Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MPG Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of N-Methylpurine DNA Glycosylase (MPG). No significant cross-reactivity or interference between N-Methylpurine DNA Glycosylase (MPG) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MPG
• Gene Full Name: N-methylpurine DNA glycosylase
• Alias Symbols: 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p;3-alkyladenine DNA glycosylase;3-methyladenine DNA glycosidase;AAG;ADPG;anpg;APNG;CRA36.1;CRA36.1 (3-methyl-adenine DNA glycosylase);DNA-3-methyladenine glycosylase;MDG;Mid1;N-methylpurine-DNA glycosylase;N-methylpurine-DNA glycosylase, MPG;PIG11;PIG16;proliferation-inducing protein 11;proliferation-inducing protein 16.
• NCBI Gene Id: 4350
• Protein Name: DNA-3-methyladenine glycosylase
• Description of Target: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
• Uniprot ID: P29372
• Protein Accession #: NP_001015052
• Nucleotide Accession #: NM_001015052.2
• Protein Size (# AA): 298