Mouse Tgfbr2 Antibody - C-terminal region (OAAB12753)

Catalog No: OAAB12753

Size:400ul

Mouse Tgfbr2 Antibody (C-term) (Cat. # OAAB12753) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Tgfbr2 antibody detected the Tgfbr2 protein (arrow).

Mouse Tgfbr2 Antibody (C-term) (Cat. # OAAB12753) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Tgfbr2 antibody detected the Tgfbr2 protein (arrow).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid.PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Function: Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non- promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non- canonical, SMAD-independent TGF-beta signaling pathways (By similarity).
  Cellular Location: Cell membrane. Single-pass type I membrane protein. Membrane raft .
  Tissue Location: Widely expressed in adult. Expressed primarily in mesenchyme and epidermis of the midgestational fetus
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Mouse Tgfbr2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-575 amino acids from the C-terminal region of mouse Tgfbr2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1,000
  Optimal dilutions should be determined by the end user.
• Reference: Droguett, R., et al. Exp. Cell Res. 316(15):2487-2503(2010)
  Robson, A., et al. Dev. Dyn. 239(9):2435-2442(2010)
  Moreno, S.G., et al. Dev. Biol. 342(1):74-84(2010)
  Ouyang, W., et al. Immunity 32(5):642-653(2010)
  Lu, L., et al. J. Immunol. 184(8):4295-4306(2010)

Target Info

• Gene Symbol: Tgfbr2
• Alias Symbols: TBR, Tbet, DNIIR, RIIDN, Tbeta, TBR-II, AU042018, TbetaRII, TbetaR-II, 1110020H15Rik
• NCBI Gene Id: 21813
• Protein Name: TGF-beta receptor type-2
• Description of Target: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
• Uniprot ID: Q62312
• Protein Accession #: NP_033397.3
• Molecular Weight: 67 kDa