Mouse Stk11 Antibody - C-terminal region (OAAB12747)

Catalog No: OAAB12747

Size:400ul

Mouse Stk11 Antibody (C-term) (Cat. # OAAB12747) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Stk11 antibody detected the Stk11 protein (arrow).

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
• Additional Information: Precautions: Mouse Stk11 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, leading to promote their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA and possibly p53/TP53 Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2: it thereby regulates inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neurons polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear. Also acts as a mediator p53/TP53- dependent apoptosis via interaction with p53/TP53: translocates to mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways
  Cellular Location: Nucleus. Cytoplasm. Membrane. Mitochondrion (By similarity). Note=Translocates to mitochondrion during apoptosis (By similarity). A small fraction localizes at membranes. Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta)
  Tissue Location: Widely expressed. Isoform 2 is predominantly expressed in testis (at protein level)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Mouse Stk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-420 amino acids from the C-terminal region of mouse Stk11.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IF: 1:25
  IHC-P: 1:25
  WB: 1:500~1:1000
  WB: 1:1000

Target Info

• Gene Symbol: Stk11
• Alias Symbols: Pa, Lkb, Lkb1, Par-4, R75140, AA408040
• NCBI Gene Id: 20869
• Protein Name: Serine/threonine-protein kinase STK11
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9WTK7
• Protein Accession #: NP_035622.1
• Molecular Weight: 49267 Da