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Datasheets/Manuals | Printable datasheet for OAAB10756 |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid. PBS with 0.09% (W/V) sodium azide. |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB, FC |
:: | Function: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN. Cellular Location: Cytoplasm. Note=Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways (By similarity). |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This Mouse Pak4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the Central region of mouse Pak4. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Predicted Homology Based on Immunogen Sequence | Human; Mouse |
Concentration | Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:1000 FC: 1:10~50 |
Gene Symbol | Pak4 |
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Alias Symbols | AW555722, mKIAA1142, 5730488L07Rik |
NCBI Gene Id | 70584 |
Protein Name | Serine/threonine-protein kinase PAK 4 |
Description of Target | Pak4 activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2 (By similarity). |
Uniprot ID | Q8BTW9 |
Protein Accession # | NP_081746.1 |
Molecular Weight | 64623 Da |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Mouse Pak4 Antibody - middle region (OAAB10756)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "Mouse Pak4 Antibody - middle region (OAAB10756)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "Mouse Pak4 Antibody - middle region (OAAB10756)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Mouse Pak4 Antibody - middle region (OAAB10756)"?
This target may also be called "AW555722, mKIAA1142, 5730488L07Rik" in publications.
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What is the shipping cost for "Mouse Pak4 Antibody - middle region (OAAB10756)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Mouse Pak4 Antibody - middle region (OAAB10756)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Mouse Pak4 Antibody - middle region (OAAB10756)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "64623 Da".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Mouse Pak4 Antibody - middle region (OAAB10756)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PAK4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PAK4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PAK4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PAK4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PAK4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PAK4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.