p27Kip1 Antibody (Phospho-Thr197) (OAAB16427)

Catalog No: OAAB16427

Size:400ul

Dot blot analysis of Mouse p27Kip1 Antibody (Phospho T197) Phospho-specific Pab (Cat. # OAAB16427) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: DB
• Additional Information: Precautions: Mouse p27Kip1 Antibody (Phospho T197) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry
  Cellular Location: Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-197, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser- 10 facilitates nuclear export. Translocate
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This mouse p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T197 of mouse p27Kip1.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: DB: 1:500

Target Info

• Gene Symbol: Cdkn1b
• Alias Symbols: p2, p27, Kip1, p27K, p27Kip1, AA408329, AI843786
• NCBI Gene Id: 12576
• Protein Name: Cyclin-dependent kinase inhibitor 1B
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P46414
• Protein Accession #: NP_034005.2
• Molecular Weight: 22193 Da