SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OASB01737 (Formerly GWB-59A430)
Size:200UG
Price: $489.00
SKU
OASB01737
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for Mouse KLRG1/MAFA Antibody : PE (OASB01737)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityMonoclonal
Clone2F1
IsotypeIgG
HostHamster
ConjugationR-PE
ApplicationFC
Additional InformationDescription: The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.
Reconstitution and StorageStore at 2-8C
ImmunogenIL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice
Concentration0.1 mg/mL
SpecificityKLRG1
CharacterizationTo ensure acceptable performance, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry: Purified antibody
Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.2 ug/106 cells
Application InfoFlow cytometry 1, Immunoprecipitation1
Other Applications DataSince applications vary, investigators are advised to determine theoptimum working dilution of the product that is appropriate for a specificapplication.
Reference1. Corral, L., T. Hanke, R.E. Vance, D. Cado, and D.H. Raulet. 2000. Eur. J. Immunol. 30:920
2. Hanke, T., L. Corral, R.E., Vance, and D.H. Raulet. 1998. 28:4409
3. Burshtyn, D., A. Scharenberg, N. Wagtmann, S. Rajagopalan, M. Peruzzi, J.- P. Kinet, and E.O. Long. 1997. Immunity 4:77
4. Nakamura, M.C., E.C. Niemi, M.J. Fisher, L.D. Schultz, W.E. Seaman, and J.C. Ryan. 1997. J. Exp. Med. 185:673
5. Liu, S., and D.H. Raulet. Personal communication.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Gene SymbolKLRG1
Gene Full NameKiller cell lectin-like receptor subfamily G member 1
Alias SymbolsMAFA, 2F1-A, 2F1-Ag, MAFA-L
NCBI Gene Id50928; 10219
Protein Namekiller cell lectin-like receptor subfamily G member 1
Uniprot IDO88713, Q96E93
Protein Accession #NP_058666.1
Nucleotide Accession #NM_016970.1
  1. What is the species homology for "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Mouse KLRG1/MAFA Antibody : PE (OASB01737)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    This target may also be called "MAFA, 2F1-A, 2F1-Ag, MAFA-L" in publications.

  5. What is the shipping cost for "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse KLRG1/MAFA Antibody : PE (OASB01737)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "KLRG1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "KLRG1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "KLRG1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "KLRG1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "KLRG1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "KLRG1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

Write Your Own Review
You're reviewing:Mouse KLRG1/MAFA Antibody : PE (OASB01737)
Your Rating