Mouse Fgfr1 Antibody - C-terminal region (OAAB11171)

Catalog No: OAAB11171

Size:400ul

Mouse Fgfr1 Antibody (C-term) (Cat. #OAAB11171) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Fgfr1 antibody detected the Fgfr1 protein (arrow).

Mouse Fgfr1 Antibody (C-term) (OAAB11171)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse adrenal glands followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Fgfr1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
  Function: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2 In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
  Cellular Location: Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus Isoform 5: Cell membrane; Single-pass type I membra
  Tissue Location: Widely expressed.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Mouse Fgfr1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 756-784 amino acids from the C-terminal region of mouse Fgfr1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
• Publications:

  Oncogenic fusion protein BCR-FGFR1 requires the breakpoint cluster region-mediated oligomerization and chaperonin Hsp90 for activation. Haematologica. 105, 1262-1273 (2020). 31439673

• Description:
  View product citations for Mouse Fgfr1 Antibody - C-terminal region (OAAB11171) on CiteAb

Target Info

• Gene Symbol: Fgfr1
• Alias Symbols: Ea, Fr, Hs, FLG, Fr1, MFR, Eask, FGFR, Flt-, Hspy, Fgfr-, Flt-2, c-fgr, FGFR-I, Fgfr-1, AW208770, bFGF-R-1
• NCBI Gene Id: 14182
• Protein Name: Fibroblast growth factor receptor 1
• Description of Target: Receptor for basic fibroblast growth factor. A shorter form of the receptor could be a receptor for FGF1 (aFGF). Receptor for FGF23 in the presence of KL.
• Uniprot ID: P16092
• Protein Accession #: NP_001073377.1
• Molecular Weight: 92 kDa