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Catalog No: OASB01701 (Formerly GWB-A15874)
Size:100UG
Price: $299.00
SKU
OASB01701
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityMonoclonal
CloneH1.2F3
IsotypeIgG
HostHamster
ConjugationR-PE-Cyanine 7
ApplicationFC
Additional InformationDescription: CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. The monoclonal antibody H1.2F3 augments PMA-induced T-cell proliferation and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
Reconstitution and StorageStore at 2-8C
ImmunogenMouse dendritic epidermal cell line Y245
Concentration0.1 mg/mL
SpecificityCD69
CharacterizationTo ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry: Fluorescein conjugate <= 1 ug/106 cells
Biotin conjugate <= 1 ug/106 cells
R-phycoerythrin conjugate <= 0.2 ug/106 cells
Allophycocyanin conjugate <= 0.2 ug/106 cells
Spectral Red conjugate <= 0.2 ug/106 cells
R-phycoerythrin-Cyanine 7 conjugate <= 0.2 ug/106 cells
Application InfoFlow cytometry 1,2,4-7, Immunoprecipitation 1, In vitro T cell and NK-cell activation 1,7
Other Applications DataSince applications vary, you should determine the optimum working dilutionfor the product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: ≤1 Ug/106 cells Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or hamster anti-mouse CD69-FITC. Large, activated cells were
Reference1. Yokoyama, W. M., F. Koning, P. J. Kehn, G. M. Pereira, G. Stingl, J. E. Coligan, and E. M. Shevach. 1988. J. Immunol. 141:369
2. Yokoyama, W.M., S.R. Maxfield, and E.M. Shevach. 1989. Immunol. Rev. 109:153
3. Zeigler, S.F., S.D. Levin, L. Johnson, N.G. Copeland, et al. 1994. J. Immunol. 152:1228
4. Ziegler, S.F., F. Ramsdell, and M.R. Alderson. 1994. Stem Cells 12:456
5. Brandle, D., S. Muller, C. Muller, H. Hengartner, and H. Pircher. 1994. Eur. J. Immunol. 24:145
6. Sobel, E. S., W. M. Yokoyama, E. M. Shevach, R. A. Eisenberg, and P. L. Cohen. 1993. J. Immunol. 150:673
7. Karlhofer, F. M. and W. M. Yokoyama. 1991. J. Immunol. 146:3662.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Gene SymbolCD69
Gene Full NameEarly activation antigen CD69
Alias SymbolsA, V, AIM, VEA, AI452015, 5830438K24Rik
NCBI Gene Id12515
Protein Nameearly activation antigen CD69
Uniprot IDP37217
Protein Accession #NP_001028294.1
Nucleotide Accession #NM_001033122.3
  1. What is the species homology for "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    This target may also be called "A, V, AIM, VEA, AI452015, 5830438K24Rik" in publications.

  5. What is the shipping cost for "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse CD69/VEA Antibody : PE/CY7 (OASB01701)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CD69"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD69"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD69"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD69"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD69"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD69"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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