Mouse CD28 Antibody : SPRD (OASB01672)

Catalog No: OASB01672 (Formerly GWB-288459)

Size:100UG

Amount Used: 0.3 Ug/106 cells BALB/c thymocytes were double-stained with hamster anti-mouse CD28-R-PE and hamster antimouse CD3ε-FITC. All cells were then gated and analyzed by two-color flow cytometry on a FACScan flow cytometer (BDIS, San Jose, CA).

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: 37.51
• Isotype: IgG
• Host: Hamster
• Conjugation: Spectral Red®
• Application: FC
• Additional Information: Description: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: C57BL/6N mouse T cell lymphoma EL-4 cell line
• Concentration: 0.1 mg/mL
• Specificity: CD28
• Characterization: To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert.
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Fluorescein conjugate <= 2 ug/10⁶ cells
  Biotin conjugate <= 2 ug/10⁶ cells
  R-phycoerythrin conjugate <= 0.2 ug/10⁶ cells
  Allophycocyanin conjugate <= 0.2 ug/10⁶ cells
  SpectralRed conjugate <= 0.2 ug/10⁶ cells
• Application Info: Flow cytometry 2,4, Immunoprecipitation 1, In vitro costimulation of T cells and NK cells 1, 4
• Other Applications Data: Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need.
• Other Applications Image 1 Data: Amount Used: 0.3 Ug/106 cells BALB/c thymocytes were double-stained with hamster anti-mouse CD28-R-PE and hamster antimouse CD3epsilon-FITC. All cells were then gated and analyzed by two-color flow cytometry on a FACScan flow cytometer (BDIS, San
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg ofpurified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing
  - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.

Target Info

• Reference: 1. Gross, J. A., E. Callas, and J. P. Allison. 1992. J. Immunol. 149:380
  2. Giese, T., J. P. Allison, and W. F. Davidson. 1993. J. Immunol. 151:597
  3. Clements, J. L., G. Winslow, C. Donahue, S. M. Cooper, J. P. Allison, and R. C. Budd. 1993. Int. Immunol. 5:1451
  4. Nandi, D., J. A. Gross, and J. P. Allison. 1994. J. Immunol. 152:3361
  5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1992. Immunol. Today 15:321
  6. Gelfanov, V., Y. G. Lai, V. Gelfanova, J. Y. Dong, J. P. Su, and N. S. Liao. 1995. J. Immunol. 155:76
  7. Harding, F. A., J. G. McArthur, J. A. Gross, D. H. Raulet, and J. P. Allison. 1992. Nature 356:607
  8. Bluestone, J.A. 1995. Immunity 2:555.
• Gene Symbol: CD28
• Gene Full Name: T-cell-specific surface glycoprotein CD28
• NCBI Gene Id: 12487
• Protein Name: T-cell-specific surface glycoprotein CD28
• Uniprot ID: P31041
• Protein Accession #: NP_031668.3
• Nucleotide Accession #: NM_007642.4