Catalog No: OASB01672 (Formerly GWB-288459)
Size:100UG
Price: $369.00
SKU
OASB01672
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for Mouse CD28 Antibody : SPRD (OASB01672)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityMonoclonal
Clone37.51
IsotypeIgG
HostHamster
ConjugationSpectral Red®
ApplicationFC
Additional InformationDescription: CD28 is a type I disulfide-linked homodimer that is constitutively expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T lymphocytes and at very low levels on NK cells. Its expression is upregulated upon T-cell activation. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) on B cells and other antigen presenting cells and plays an important role in the interaction between T cells and B cells. CD28 is a costimulatory receptor involved in many, but not all, T-cell independent immune responses.
Reconstitution and StorageStore at 2-8C
ImmunogenC57BL/6N mouse T cell lymphoma EL-4 cell line
Concentration0.1 mg/mL
SpecificityCD28
CharacterizationTo ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry: Fluorescein conjugate <= 2 ug/106 cells
Biotin conjugate <= 2 ug/106 cells
R-phycoerythrin conjugate <= 0.2 ug/106 cells
Allophycocyanin conjugate <= 0.2 ug/106 cells
SpectralRed conjugate <= 0.2 ug/106 cells
Application InfoFlow cytometry 2,4, Immunoprecipitation 1, In vitro costimulation of T cells and NK cells 1, 4
Other Applications DataSince applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: 0.3 Ug/106 cells BALB/c thymocytes were double-stained with hamster anti-mouse CD28-R-PE and hamster antimouse CD3epsilon-FITC. All cells were then gated and analyzed by two-color flow cytometry on a FACScan flow cytometer (BDIS, San
Storage- The purified (UNLB) antibody is supplied as 0.5 mg ofpurified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing
- The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Reference1. Gross, J. A., E. Callas, and J. P. Allison. 1992. J. Immunol. 149:380
2. Giese, T., J. P. Allison, and W. F. Davidson. 1993. J. Immunol. 151:597
3. Clements, J. L., G. Winslow, C. Donahue, S. M. Cooper, J. P. Allison, and R. C. Budd. 1993. Int. Immunol. 5:1451
4. Nandi, D., J. A. Gross, and J. P. Allison. 1994. J. Immunol. 152:3361
5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1992. Immunol. Today 15:321
6. Gelfanov, V., Y. G. Lai, V. Gelfanova, J. Y. Dong, J. P. Su, and N. S. Liao. 1995. J. Immunol. 155:76
7. Harding, F. A., J. G. McArthur, J. A. Gross, D. H. Raulet, and J. P. Allison. 1992. Nature 356:607
8. Bluestone, J.A. 1995. Immunity 2:555.
Gene SymbolCD28
Gene Full NameT-cell-specific surface glycoprotein CD28
NCBI Gene Id12487
Protein NameT-cell-specific surface glycoprotein CD28
Uniprot IDP31041
Protein Accession #NP_031668.3
Nucleotide Accession #NM_007642.4
  1. What is the species homology for "Mouse CD28 Antibody : SPRD (OASB01672)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Mouse CD28 Antibody : SPRD (OASB01672)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Mouse CD28 Antibody : SPRD (OASB01672)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse CD28 Antibody : SPRD (OASB01672)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "Mouse CD28 Antibody : SPRD (OASB01672)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse CD28 Antibody : SPRD (OASB01672)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse CD28 Antibody : SPRD (OASB01672)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse CD28 Antibody : SPRD (OASB01672)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CD28"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD28"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD28"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD28"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD28"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD28"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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