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Catalog No: OASB01690 (Formerly GWB-65030E)
Size:500UG
Price: $409.00
SKU
OASB01690
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for Mouse CD154/CD40L Antibody : FITC (OASB01690)
Product Info
Tested Species ReactivityMouse
Predicted Species ReactivityMouse
ClonalityMonoclonal
CloneMR1
IsotypeIgG
HostHamster
ConjugationFITC
ApplicationFC, IHC-F
Additional InformationDescription: CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. It is an important accessory molecule in T cell-B cell costimulatory interactions and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporin-sensitive. The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function. Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens yet does not alter the immune response to TI antigens.
Reconstitution and StorageStore at 2-8C
ImmunogenActivated mouse Th1 clone D1.6
Concentration0.5 mg/mL
SpecificityCD154
CharacterizationTo ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry: Fluorescein conjugate <= 2 ug/106 cells
Biotin conjugate <= 2 ug/106 cells
R-phycoerythrin conjugate <= 0.2 ug/106 cells
Application InfoFlow cytometry 1, 5, Immunoprecipitation 1, In vivo and in vitro blockage of CD154 function 1, 3, 5
Other Applications DataSince applications vary, you should determine the optimum working dilutionfor the product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: 2 Ug/106 cells Partially purified spleen T cells from BALB/c mice were incubated with either hamster IgG or platebound hamster anti-mouse CD3epsilon for 7 hours at 37 C. The cells were then harvested, double-stained with anti-CD154/CD
Reference1. Noelle, R.J., M. Roy, D.M. Shepherd, I Stamenkovic, J.A. Ledbetter, and A. Aruffo. 1992. Proc. Natl. Acad. Sci. U.S.A. 89:6550
2. Aruffo, A., M. Farrington, D. Hollenbaugh, X. Li, A. Milatovich, S. Nonoyama, J. Bajorath, L.S. Grosmaire, R. Stenkamp, and M. Neubauer
1993. Cell 72:291
3. Hollenbaugh, D., L.S. Grosmaire, C.D. Kullas, N.J. Chalupny, S. Braesch- Andersen, R.J. Noelle, I. Stamenkovic, J.A. Ledbetter, and A. Aruffo
1992. EMBO J. 11:4313
4. Foy, TM., DM Shepherd, F.H. Durie, A. Aruffo, J.A. Ledbeter, and R.J. Noelle. 1993. J. Exp. Med. 178:1567
5. Foy, T.M., D.M. page, T.J. Waldschmidt, A. Schoneveld, J.D. Laman, S.R. Masters, L. Tygrett, J.A. Ledbetter, A. Aruffo, and E. Claassen
1995. J. Exp. Med. 182:1377
6. Roy, M., T.J. Waldschmidt, A. Aruffo, J.A. Ledbetter, and R.J. Noelle. 1993. J. Immunol. 151:2497
7. Armitage, R.J., W.C. Fanslow, L. Stockbine, T.A. Sato, K.N. Clifford, B.M. Macduff, D.M. Anderson, S.D. Gimpel, T. Davis- Smith, and C.R
Maliszewski. 1992. Nature 357:80.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Gene SymbolCD40LG
Gene Full NameCD40 ligand
Alias SymbolsIGM, IMD, gp3, Cd40, HIGM, IMD3, Ly-6, Ly62, T-BA, TRAP, Tnfs, gp39, CD154, Cd40l, HIGM1, Ly-62, T-BAM, CD40-L, Tnfsf5, Tnlg8b
NCBI Gene Id21947
Protein NameCD40 ligand
Uniprot IDP27548
Protein Accession #NP_035746.2
Nucleotide Accession #NM_011616.2
  1. What is the species homology for "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".

  2. How long will it take to receive "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Mouse CD154/CD40L Antibody : FITC (OASB01690)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    This target may also be called "IGM, IMD, gp3, Cd40, HIGM, IMD3, Ly-6, Ly62, T-BA, TRAP, Tnfs, gp39, CD154, Cd40l, HIGM1, Ly-62, T-BAM, CD40-L, Tnfsf5, Tnlg8b" in publications.

  5. What is the shipping cost for "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse CD154/CD40L Antibody : FITC (OASB01690)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CD40LG"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD40LG"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD40LG"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD40LG"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD40LG"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD40LG"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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