Mouse Camk2d Antibody - middle region (OAAB10098)

Catalog No: OAAB10098

Size:400ul

Mouse Camk2d Antibody (Center) (Cat. #OAAB10098) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Camk2d antibody detected the Camk2d protein (arrow).

Western blot analysis of Camk2d (arrow) using rabbit polyclonal Mouse Camk2d Antibody (Center) (Cat. #OAAB10098). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Camk2d gene.

Mouse Camk2d Antibody (Center) (OAAB10098)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Camk2d Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Zebrafish
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: Mouse Camk2d Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser- 2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor- coupling factor
  Cellular Location: Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side (Probable). Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (Probable)
  Tissue Location: Expressed in cardiac muscle and skeletal muscle. Isoform Delta 2, isoform Delta 6, isoform Delta 6 and isoform Delta 10 are expressed in cardiac muscle. Isoform Delta 2 is expressed in skeletal muscle
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Mouse Camk2d antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the Central region of mouse Camk2d.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Pig; Rabbit; Rat; Zebrafish
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: Camk2d
• Alias Symbols: CaMK II, [d]-CaMKII, 2810011D23Rik, 8030469K03Rik
• NCBI Gene Id: 108058
• Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit delta
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q6PHZ2
• Protein Accession #: NP_001020609.1
• Molecular Weight: 56369 Da