Size:500 ug
$315.00
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SKU
OASB00777
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More Information
Tested Species Reactivity Rat
Clonality Monoclonal
Clone 2B10A8
Isotype IgG2b
Host Mouse
Conjugation FITC: Fluorescein Isothiocyanate
Application FC, FLISA, IHC-F, WB
Reconstitution and Storage Store at 2-8C
Immunogen Rat IgG2b hybridoma
Concentration 0.5 mg/mL
Datasheets/Manuals Printable datasheet for anti-Igh-3 (OASB00777) antibody
Specificity IgG2b
Characterization To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested as a second step reagent by flowcytometry and/or ELISA to conform to characteristics of a standard reference reagent. Representative dataare included in this product insert.
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eyeor face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact aphysician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in runningwater before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry:
ELISA:
Fluorescein and Cy5 conjugates
Biotin conjugate
BIMA conjugate
R-phycoerythrin conjugate
Horseradish peroxidase conjugate
Alkaline Phosphatase conjugate
<=0.3 ug/106 cells
<=0.3 ug/106 cells
<=0.3 ug/106 cells
<=0.02 ug/106 cells
1:4,000-1:8,000
1:2,000-1:4,000
Application Info Identification and enumeration of IgG2b+ cells by immunofluorescence, Second step reagent for rat IgG2b monoclonal antibodies, ELISA
Other Applications Data Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds.
Other Applications Image 1 Data Amount Used: 0.3 Ug/106 cells BALB/c splenocytes were incubated with rat IgG2b isotype, rat anti-mouse CD22 (2D6, rat IgG1), rat anti-mouse CD8 (53-6.7, rat IgG2a), rat anti-mouse CD45(LCA) (I3/2.3, rat IgG2b), rat anti-mouse CD24 (30-F1, rat IgG
Storage - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg and the Cyanine 5 (Cy5) conjugate is supplied as 0.1mg in1.0 mL of PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50%Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze!
- The biotin- maleimide (BIMA) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Official Gene Full Name Ig gamma-2B chain C region
Swissprot Id P20761
  1. What is the species homology for "Rat IgG2b Antibody : FITC (OASB00777)"?

    The tested species reactivity for this item is "Rat". This antibody is predicted to have homology to "".

  2. How long will it take to receive "Rat IgG2b Antibody : FITC (OASB00777)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Rat IgG2b Antibody : FITC (OASB00777)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Rat IgG2b Antibody : FITC (OASB00777)"?

    This target may also be called "" in publications.

  5. What is the shipping cost for "Rat IgG2b Antibody : FITC (OASB00777)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Rat IgG2b Antibody : FITC (OASB00777)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Rat IgG2b Antibody : FITC (OASB00777)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Rat IgG2b Antibody : FITC (OASB00777)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for ""?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with ""?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with ""?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with ""?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with ""?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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