- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Human MMP-9 Antibody (OASB01327) |
---|
Tested Species Reactivity | Human |
---|---|
Predicted Species Reactivity | Human |
Clonality | Monoclonal |
Clone | SB15c |
Isotype | IgG2a |
Host | Mouse |
Conjugation | Unconjugated |
Application | IHC-P, ICC, WB |
Additional Information | Description: Matrix metalloproteinases (MMPs) are a family of at least 23 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-9 has shown substrate specificity to collagens types IV, V, XI, and XIV as well as additional extracellular matrix components including gelatin, elastin, vitronectin, and laminin. |
Reconstitution and Storage | Store at 2-8C |
Immunogen | Recombinant full length MMP-9 |
Concentration | 0.5 mg/mL |
Specificity | MMP-9 |
Characterization | To insure lot- to- lot consistency, each batch of product is tested by ELISA to conform with thecharacteristics of a standard reference reagent. |
Dilution | ELISA: Purified antibody HRP conjugate 1:3,000-1:8,000 1:1,000-1:4,000 Immunohistochemistry: Purified antibody HRP conjugate <= 2 ug/mL 1:1,000-1:2,000 |
Application Info | Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunohistochemistry (frozen and paraffin embedded sections) |
Other Applications Data | Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need. |
Storage | - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50%glycerol/50% PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - Reagents are stable for the period shown on the label if stored as directed. |
Reference | 1. Steffensen, B., U.M. Wallon, and C.M. Overall. 1995. J. Biol. Chem. 270:11555 2. Allan, J.A., A.J. Docherty, P.J. Barker, N.S. Huskisson, J.J. Reynolds, and G. Murphy. 1995. Biochem. J. 309:229 3. Murphy, G., M.I. Cockett, R.V. Ward, and A.J. Docherty. 1991. J. Biol. Chem. 277:277 4. Murphy, G., J.J. Reynolds, U. Bretz, and M. Baggiolini. 1982. Biochem. J. 203:209 5. Welgus, H.G., C.J. Fliszar, J.L. Seltzer, T.M. Schmid, and J.J. Jeffery. 1990. J. Biol. Chem. 265:13521 6. Sires, U.I., B. Dublet, E. Aubert- Foucher, M. van der Rest, and H.G. Welgus. 1995. J. Bio. Chem. 270:1062 7. Patterson, B.C., and Q.A. Sang. 1997. J. Biol. Chem. 272:28823 8. Fosang, A.J., P.J. Neame, K. Last, T.E. Hardingham, G. Murphy, and J.A. Hamilton. 1992. J. Biol. Chem. 267:19470 9. Ochieng, J., R. Fridman, P. Nangia- Makker, D.E. Kleiner, L.A. Liotta, W.G. Stetler- Stevenson, and A. Raz. 1994. Biochemistry 33:14109 10. Sires, U.I., G.L. Griffin, T.J. Brekelmann, R.P. Mecham, G. Murphy, A.E. Chung, H.G. Welgus, and R.M. Senior. 1993. J. Biol. Chem. 268:2069 11. Ashworth, J.L., G. Murphy, M.J. Rock, M.J. Sherratt, S.D. Shapiro, C.A. Shuttleworth, and C.M. Kielty. 1999. Biochem. J. 340:171 12. Lelongt, B., S. Bengatta, M. Delauche, L.R. Lund, Z. Werb, and P.M. Ronco. 2001. J. Exp Med. 193:793 13. Ito, A., A. Mukaiyama, Y. Itoh, H. Nagase, I.B. Thogersen, J.J. Enghild, Y. Sasaguri, and Y. Mori. 1996. J. Biol. Chem. 271:14657 14. Chandler, S., R. Coates, A. Gearing, J. Lury, G. Wells, and E. Bone. 1995. Neurosci. Lett. 201:223. |
Gene Symbol | MMP9 |
---|---|
Gene Full Name | Matrix metalloproteinase-9 |
Alias Symbols | GELB, CLG4B, MMP-9, MANDP2 |
NCBI Gene Id | 4318; 17395 |
Protein Name | matrix metalloproteinase-9 |
Description of Target | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. |
Uniprot ID | P14780, P41245 |
Protein Accession # | NP_004985.2 |
Nucleotide Accession # | NM_004994.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Human MMP-9 Antibody (OASB01327)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
-
How long will it take to receive "Human MMP-9 Antibody (OASB01327)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
-
What buffer format is "Human MMP-9 Antibody (OASB01327)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Human MMP-9 Antibody (OASB01327)"?
This target may also be called "GELB, CLG4B, MMP-9, MANDP2" in publications.
-
What is the shipping cost for "Human MMP-9 Antibody (OASB01327)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Human MMP-9 Antibody (OASB01327)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Human MMP-9 Antibody (OASB01327)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Human MMP-9 Antibody (OASB01327)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "MMP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "MMP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "MMP9"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "MMP9"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "MMP9"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "MMP9"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.