Human MMP-9 Antibody : HRP (OASB01328)

Catalog No: OASB01328

Size:1ML

Amount Used: 5Ug/mL Paraffin embedded sections of human colon were stained with Cat. No. 12025-01. Goat Anti-Mouse IgG2a, human ads-BIOT and Streptavidin-HRP were used and then developed with DAB.

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG2a-HRP isotype control and Mouse Anti-Human MMP-9-HRP.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: SB15c
• Isotype: IgG2a
• Host: Mouse
• Conjugation: HRP
• Application: IHC-P, ICC, WB
• Additional Information: Description: Matrix metalloproteinases (MMPs) are a family of at least 23 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-9 has shown substrate specificity to collagens types IV, V, XI, and XIV as well as additional extracellular matrix components including gelatin, elastin, vitronectin, and laminin.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Recombinant full length MMP-9
• Concentration: Lot specific
• Specificity: MMP-9
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA to conform with thecharacteristics of a standard reference reagent.
• Dilution: ELISA: Purified antibody
  HRP conjugate
  1:3,000-1:8,000
  1:1,000-1:4,000
  Immunohistochemistry: Purified antibody
  HRP conjugate
  <= 2 ug/mL
  1:1,000-1:2,000
• Application Info: Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunohistochemistry (frozen and paraffin embedded sections)
• Other Applications Data: Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
• Storage: - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50%glycerol/50% PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - Reagents are stable for the period shown on the label if stored as directed.
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Target Info

• Gene Symbol: MMP9
• Gene Full Name: Matrix metalloproteinase-9
• Alias Symbols: GELB, CLG4B, MMP-9, MANDP2
• NCBI Gene Id: 4318; 17395
• Protein Name: matrix metalloproteinase-9
• Description of Target: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Uniprot ID: P14780, P41245
• Protein Accession #: NP_004985.2
• Nucleotide Accession #: NM_004994.2