Human MMP-3 Antibody : HRP (OASB01326)

Catalog No: OASB01326

Size:1ML

Amount Used: 5mg/mL Paraffin embedded sections of human colon carcinoma were stained with Cat. No. 12020-01. Goat Anti-Mouse IgG2a, human ads-BIOT and Streptavidin-HRP were used and then developed with DAB.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: SB14d
• Isotype: IgG2a
• Host: Mouse
• Conjugation: HRP
• Application: IHC-P, IHC-F, ICC
• Additional Information: Description: Matrix metalloproteinases (MMPs) are a family of at least 23 structurally related, zinc-containing enzymes that have the ability to breakdown connective tissue. MMP-3 has shown substrate specificity to collagens types I, II, III, IV, VI, IX, X, and XIV as well as additional extracellular matrix components including gelatin, fibronectin, aggrecan, and perlecan.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Recombinant MMP-3
• Concentration: Lot specific
• Specificity: MMP-3
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by ELISA to conform with thecharacteristics of a standard reference reagent.
• Dilution: ELISA: Purified antibody
  HRP conjugate
  1:3,000-1:8,000
  1:1,000-1:4,000
  Immunohistochemistry: Purified antibody
  HRP conjugate
  £ 2 mg/mL
  1:1,000-1:2,000
• Application Info: Enzyme-Linked-Immunosorbent-Assay (ELISA), Immunohistochemistry (frozen and paraffin embedded sections)
• Other Applications Data: Since applications vary, you should determine the optimum working dilutionof the product that is appropriate for your specific need.
• Storage: · The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  · The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50%glycerol/50% PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  · Reagents are stable for the period shown on the label if stored as directed.
• Reference: 1. Nicholson, R., G. Murphy, and R. Breathnach. 1989. Biochemistry 28:5195
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  4. Chin, J.R., G. Murphy, and Z. Werb. 1985. J. Biol. Chem. 260:12367
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  6. Bini, A., Y. Itoh, B.J. Kudryk, and H. Nagase. 1996. Biochemistry 35:13056
  7. Lijnen, H.R., F. Ugwu, A. Bini, and D. Collen. 1998. Biochemistry 37:4699
  8. Sires, U.I., G.L. Griffin, T.J. Broekelmann, R.P. Mecham, G. Murphy, A.E. Ching, H.G. Welhus, and R.M. Senior. 1993. J. Biol. Chem
  268:2069
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Target Info

• Gene Symbol: MMP3
• Gene Full Name: Stromelysin-1
• Alias Symbols: SL-1, STMY, STR1, CHDS6, MMP-3, STMY1
• NCBI Gene Id: 4314
• Protein Name: stromelysin-1
• Description of Target: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
• Uniprot ID: P08254
• Protein Accession #: NP_002413.1
• Nucleotide Accession #: NM_002422.4