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Datasheets/Manuals | Printable datasheet for Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Clonality | Monoclonal |
Clone | SB46b |
Isotype | IgG2a |
Host | Mouse |
Conjugation | Biotin |
Application | WB |
Additional Information | Description: Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens, and differentiation-promoting agents via a protein kinase cascade. Erk1 and Erk2 are ubiquitous and abundant although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding. Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. Both sites must be phosphorylated for maximum activity. These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. They target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus. The SB46b monoclonal antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively. |
Reconstitution and Storage | Store at 2-8C |
Immunogen | Recombinant human Erk1 and Erk2 |
Concentration | 0.5 mg/mL |
Specificity | ERK1/2 |
Characterization | To ensure acceptable performance, each batch of product is tested by Western blotting to conform tocharacteristics of a standard reference reagent. |
Warning | Immunoconjugates contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes,or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoicacid under acidic conditions. Dilute azide- containing compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in lead or copper plumbing. |
Dilution | Western Blotting: Purified Antibody 1-2ug/mL Immunoprecipitation: Purified Antibody 10-15ug |
Storage | - The purified (UNLB) antibody is supplied as 0.05 mg purified immunoglobulin in 0.1 mL of 100 mM boratebuffered saline, pH 8.0; store at 2- 8 C - HRP conjugate is supplied in 1.0 mL of stock solution in 50% glycerol/50% phosphate buffered saline, pH.7.4 No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - Reagents are stable for the period shown on the label if stored as directed. |
Reference | 1) Aviva Systems Biology, Inc. Unpublished 2) Boulton, T.G. et al. (1990) Science 249: 64- 67 3) Charest, D.L. et al. (1993) Mol Cell. Biol 13: 4679- 4690 4) Hardie, G. amd S. Hanks (1995) The Protein Kinase Facts Book, Protein- Serine Kinases, Academic Press Limited, San Diego 418pps 5) Pearson, G. etal. (2001) Endoc. Rev. 22(2): 153- 183 6) Xu, B. et al. (2001) J. Biol. Chem. 276(28): 26509- 26515. |
Gene Symbol | MAPK3, MAPK1 |
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Gene Full Name | Mitogen-activated protein kinase 3 |
Alias Symbols | ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK |
NCBI Gene Id | 5595; 5594 |
Protein Name | mitogen-activated protein kinase 3 |
Description of Target | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. |
Uniprot ID | P27361, P28482 |
Protein Accession # | NP_001035145.1 |
Nucleotide Accession # | NM_001040056.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
This target may also be called "ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK" in publications.
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What is the shipping cost for "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Human Erk1/2 (MAPK) Antibody : Biotin (OASB01339)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "MAPK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "MAPK3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "MAPK3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "MAPK3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "MAPK3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "MAPK3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.