Human CD8 Antibody : PE (OASB01016)

Catalog No: OASB01016

Size:100T

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD8-PE and Mouse Anti-Human CD4-FITC.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: UCHT4
• Isotype: IgG2a
• Host: Mouse
• Conjugation: R-PE
• Application: FC
• Additional Information: Description: CD8 is a 33 kDa transmembrane glycoprotein expressed as either a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer. It is expressed on the "cytotoxic/suppressor" subpopulation of peripheral T cells. CD8 functions primarily as a coreceptor with MHC class I-restricted TCR's in antigen recognition.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Thymocytes and Sezary T cells
• Concentration: Lot specific
• Specificity: CD8
• Characterization: To insure lot to lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Purified (UNLB) antibody
  Fluorescein conjugate
  R-phycoerythrin conjugate
  Spectral Red conjugate
  <= 1 ug/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
• Application Info: Flow cytometry, Immunohistochemistry (frozen sections), Immunoprecipitation
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent
  Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze!
  - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected.
• Reference: 1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD8 Section, Academic Press, New York, p. 149
  2. Zamoyska, R. 1994. Immunity 1:243.

Target Info

• Gene Symbol: CD8A
• Gene Full Name: T-cell surface glycoprotein CD8 alpha chain
• Alias Symbols: CD8, p32, Leu2
• NCBI Gene Id: 925; 698329; 102124337; 735755
• Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Description of Target: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P01732, F7DXL7
• Protein Accession #: NP_001139345.1
• Nucleotide Accession #: NM_001145873.1