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Datasheets/Manuals | Printable datasheet for Human CD45/LCA Antibody (OASB01102) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Clonality | Monoclonal |
Clone | 2D1 |
Isotype | IgG1 |
Host | Mouse |
Conjugation | Unconjugated |
Application | FC, IHC-F, IHC-P, IP, WB |
Additional Information | Description: CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation. |
Reconstitution and Storage | Store at 2-8C |
Immunogen | Human peripheral blood mononuclear cells |
Concentration | 0.1 mg/mL |
Specificity | CD45 |
Characterization | To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. |
Dilution | Flow Cytometry: Purified (UNLB) antibody <= 1 ug/106 cells |
Application Info | Flow cytometry |
Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
Storage | - The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C. |
Reference | 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, OxfordUniversity Press, Oxford 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD45 Section, Academic Press, New York, p. 244 3. Dalchau, R., J. Kirkley, and J.W. Fabre. Eur. J. Immunol. 10:737 4. Thomas, M.L. 1989. Annu. Rev. Immunol. 7:339 5. Trowbridge, I.S., and M.L. Thomas. 1994. Annu. Rev. Immunol. 12:85. |
Gene Symbol | PTPRC |
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Gene Full Name | Receptor-type tyrosine-protein phosphatase C |
Alias Symbols | LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180 |
NCBI Gene Id | 5788 |
Protein Name | receptor-type tyrosine-protein phosphatase C |
Description of Target | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. |
Uniprot ID | P08575 |
Protein Accession # | NP_002829.3 |
Nucleotide Accession # | NM_002838.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Human CD45/LCA Antibody (OASB01102)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "Human CD45/LCA Antibody (OASB01102)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Human CD45/LCA Antibody (OASB01102)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Human CD45/LCA Antibody (OASB01102)"?
This target may also be called "LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180" in publications.
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What is the shipping cost for "Human CD45/LCA Antibody (OASB01102)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Human CD45/LCA Antibody (OASB01102)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Human CD45/LCA Antibody (OASB01102)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Human CD45/LCA Antibody (OASB01102)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PTPRC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PTPRC"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PTPRC"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PTPRC"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PTPRC"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PTPRC"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.