Human CD16 Antibody (OASB01069)

Catalog No: OASB01069

Size:100UG

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: GRM1
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Unconjugated
• Application: FC, IHC-F, IP, WB, CMCD
• Additional Information: Description: CD16, a member of the immunoglobulin superfamily, is a 50-65 kDa glycoprotein found as both a transmembrane and GPI-linked form. The transmembrane form of CD16 is expressed on NK cells, granulocytes, macrophages, and mast cells but not on eosinophils. The GPI-anchored type of CD16 is found only on neutrophils. CD16 is involved in NK activation and signal transduction.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Mononuclear cells from a prolymphocytic B-leukemia
• Concentration: 0.1 mg/mL
• Specificity: CD16
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform with thecharacteristics of a standard reference reagent.
• Warning: Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Flow Cytometry: Purified (UNLB) antibody
  Fluorescein conjugate
  Biotin conjugate
  R-phycoerythrin conjugate
  Spectral Red conjugate
  <= 1 ug/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
  10 uL/10⁶ cells
• Application Info: Flow cytometry, Immunohistochemistry (frozen sections), Western blotting, Immunoprecipitation
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.0. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze!
  - The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze!
  - Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored asdirected.
• Reference: 1. McMichael, A.K., P.C.L. Beverly, S. Cobbold, M.J. Crumpton, W. Gilks, F.M. Gotch, N. Hogg, M. Horton, N. Ling, I.C.M. MacLennan, D.Y
  Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann, eds. 1987. Leukocyte Typing III: White Cell Differentiation Antigens, OxfordUniversity Press, Oxford
  2. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: WhiteCell Differentiation Antigens, Oxford University Press, Oxford
  3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte AntigensFacts Book, 2nd Edition, CD16 Section, Academic Press, New York, p. 173
  4. Ravetch, J.V., and B. Perussia. 1989. J. Exp. Med. 170:481
  5. Takai, T., M. Li, D. Sylvestre, R. Clynes, and J.V. Ravetch. 1994. Cell 76:519.

Target Info

• Gene Symbol: FCGR3A
• Gene Full Name: Low affinity immunoglobulin gamma Fc region receptor III-A
• Alias Symbols: CD16, FCG3, CD16A, FCGR3, IGFR3, IMD20, FCR-10, FCRIII, FCGRIII, FCRIIIA
• NCBI Gene Id: 2214
• Protein Name: low affinity immunoglobulin gamma Fc region receptor III-A
• Description of Target: This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P08637
• Protein Accession #: NP_000560.6
• Nucleotide Accession #: NM_000569.7