Cytokeratin 8 Antibody : HRP (OASB01188)

Catalog No: OASB01188

Size:1ML

DILUTION: 10Ug/ml Cell Cytospin of the HCC38 pancreatic carcinoma cell line was incubated with anti-cytokeratin 8-FITC monoclonal antibody. Positive cells were viewed by fluorescence microscopy.

Paraffin embedded human gastric cancer tissue was stained with Mouse IgG2a-HRP isotype control and Mouse Anti-Cytokeratin 8-HRP followed by DAB and hematoxylin.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: SB37b
• Isotype: IgG2a
• Host: Mouse
• Conjugation: HRP
• Application: ICC, IHC-P, FC, WB, IP
• Additional Information: Description: Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology. Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 8 belongs to the type B (basic) subfamily of keratins and exists in combination with keratin 18. It is expressed in all simple type epithelia tissues (e.g., liver, pancreas, kidney, gut epithelial lining) but not in stratified squamous epithelia. Cytokeratin 8 is also present in the majority of adenocarcinomas and ductal carcinomas but is absent in most squamous cell carcinomas.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Recombinant C-terminal cytokeratin 8
• Concentration: 0.1 mg/mL
• Specificity: Cytokeratin 8
• Characterization: To ensure acceptable performance, each batch of product is tested by ELISA, immunohistochemical staining(HT29 and HCC38 cells), or Western blotting (HT29 and HCC38 cell lysates) to conform to characteristics of astandard reference reagent.
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes,or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoicacid under acidic conditions. Dilute azide- containing compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in lead or copper plumbing.
• Dilution: Immunohistochemistry: Purified Antibody
  FITC Conjugate
  HRP
  10ug/mL
  10ug/mL
  1:1000-1:2000
  Western Blotting: Purified Antibody
  HRP
  1-2ug/mL
  1:5,000-1:10,000
• Application Info: ELISA, Immunohistochemistry (FS or cultured and methanol-fixed cell lines), Western blotting
• Storage: - The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2; store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.1 mg in 0.2 mL PBS/NaN3. Store at 2- 8 C
  - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS,pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
  - Reagents are stable for the period shown on the label if stored as directed.
• Reference: 1) Aviva Systems Biology, Inc. Unpublished
  2) Lodish, H. et al. (2000) In: Molecular Cell Biology. NY, NY, W.H. Freeman and Co., pp 795- 847
  3) Fuchs, E.V. et al. (1981) Cell 27: 75- 84
  4) Hanukoglu, I. And E. Fuchs. (1983) Cell 33: 915- 924
  5) Schiller, D.L. et al. (1982) EMBO J. 6:761- 769
  6) Tseng, S.C.G. et al. (1982) Cell 30: 361- 372.

Target Info

• Gene Symbol: KRT8
• Gene Full Name: Keratin, type II cytoskeletal 8
• Alias Symbols: K8, KO, CK8, CK-8, CYK8, K2C8, CARD2
• NCBI Gene Id: 3856; 16691
• Protein Name: keratin, type II cytoskeletal 8
• Description of Target: This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
• Uniprot ID: P05787, P11679
• Protein Accession #: NP_001243222.1
• Nucleotide Accession #: NM_001256293.1