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Monkey IgM Antibody - FITC Conjugated (OARA04821)

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Description of Target:
Anti-Monkey IgM (mu chain) Fluorescein antibody generated in goat specifically detects monkey IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Monkey IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
Molecular Weight:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Monkey IgM whole molecule
Product Format:
Lyophilized 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2), 10 mg/mL BSA (immunoglobulin & protease free) with 0.01% (w/v) Sodium Azide
Color: Green
Reconstitution and Storage:
Store vial at 4°C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
1.0 mg/ml (by UV absorbance at 280 nm) (prior to lyophilization)
Printable datasheet for OARA04821
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against Monkey IgG or Monkey IgA. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other Monkey heavy or light chain isotypes.
Fluorescein (FITC)
Application Info:
FLISA: 1:10000~50000
IF: 1:1000~5000
This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Additional Information:
Emission Wavelength: 528
Excitation Wavelength: 495
Fluorochrome/Protein Ratio: 3.32
Conjugation Chemistry: Isothiocyanate

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