RAGE ELISA kit (Human)

Catalog No: OKCD00530 (Formerly GWB-KBBNF2)

Size:96WELLS

Human MOK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renal Tumor Antigen (RAGE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renal Tumor Antigen (RAGE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renal Tumor Antigen (RAGE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Renal Tumor Antigen (RAGE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-MOK Microplate: 96 Wells (12 x 8 Well Strips)
  • MOK Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated MOK Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.062 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Renal Tumor Antigen (RAGE). No significant cross-reactivity or interference between Renal Tumor Antigen (RAGE) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MOK
• Gene Full Name: MOK protein kinase
• Alias Symbols: MAPK/MAK/MRK overlapping kinase;MOK protein kinase;RAGE;RAGE1;RAGE-1;renal cell carcinoma antigen;renal tumor antigen 1;STK30.
• NCBI Gene Id: 5891
• Protein Name: Q9UQ07
• Description of Target: MAPK/MAK/MRK overlapping kinase
• Uniprot ID: https://www.uniprot.org/uniprot/Q9UQ07
• Protein Accession #: NP_001258940
• Nucleotide Accession #: NM_001272011.1
• Protein Size (# AA): 419