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Catalog No: OKCD00530 (Formerly GWB-KBBNF2)
Price: $500.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsPrintable datasheet for RAGE ELISA kit (Human)
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renal Tumor Antigen (RAGE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Renal Tumor Antigen (RAGE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Renal Tumor Antigen (RAGE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Renal Tumor Antigen (RAGE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renal Tumor Antigen (RAGE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-MOK Microplate96 Wells (12 x 8 Well Strips)
MOK Lyophilized Standard2 x 10 ng
100X Biotinylated MOK Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.062 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Renal Tumor Antigen (RAGE). No significant cross-reactivity or interference between Renal Tumor Antigen (RAGE) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolMOK
Gene Full NameMOK protein kinase
Alias SymbolsMAPK/MAK/MRK overlapping kinase;MOK protein kinase;RAGE;RAGE1;RAGE-1;renal cell carcinoma antigen;renal tumor antigen 1;STK30.
NCBI Gene Id5891
Protein NameQ9UQ07
Description of TargetMAPK/MAK/MRK overlapping kinase
Uniprot ID
Protein Accession #NP_001258940
Nucleotide Accession #NM_001272011.1
Protein Size (# AA)419
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