MOG ELISA Kit (Rat) (OKCD00239)

Catalog No: OKCD00239 (Formerly GWB-KBBUT9)

Size:96WELLS

Rat Mog ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Oligodendrocyte Glycoprotein (MOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Oligodendrocyte Glycoprotein (MOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Oligodendrocyte Glycoprotein (MOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Myelin Oligodendrocyte Glycoprotein (MOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Oligodendrocyte Glycoprotein (MOG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Oligodendrocyte Glycoprotein (MOG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Mog Microplate: 96 Wells (12 x 8 Well Strips)
  • Mog Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Mog Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myelin Oligodendrocyte Glycoprotein (MOG). No significant cross-reactivity or interference between Myelin Oligodendrocyte Glycoprotein (MOG) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: MOG
• Gene Full Name: myelin oligodendrocyte glycoprotein
• Alias Symbols: Myelin-oligodendrocyte glycoprotein
• NCBI Gene Id: 24558
• Protein Name: Myelin-oligodendrocyte glycoprotein
• Description of Target: Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
• Uniprot ID: Q63345
• Protein Accession #: NP_073159
• Nucleotide Accession #: NM_022668.2
• Protein Size (# AA): 245