Catalog No: OKEH02306
Size:96T
Price: $525.00
SKU
OKEH02306
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MOCOS ELISA Kit (Human) (OKEH02306) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MOCOS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MOCOS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MOCOS captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.3% (n = 20)
Mean Inter-assay CV%: < 11.3% (n = 20)
ELISA Kit Component
ComponentAmount
MOCOS Microplate96 Wells (12 x 8 Well strips)
MOCOS Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MOCOS Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.08 ng/mL
SpecificityNatural and recombinant Human Molybdenum cofactor sulfurase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMOCOS
Gene Full Namemolybdenum cofactor sulfurase
Alias SymbolsHMCS;MCS;molybdenum cofactor sulfurase;Molybdenum cofactor sulfurtransferase;MOS.
NCBI Gene Id55034
Protein NameQ96EN8
Description of TargetMolybdenum cofactor sulfurase
Uniprot IDhttps://www.uniprot.org/uniprot/Q96EN8
Protein Accession #NP_060417.2
Nucleotide Accession #NM_017947.2
Protein Size (# AA)888
Molecular Weight98 kDa
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