MOB4A Antibody - C-terminal region (OAAB16768)

Catalog No: OAAB16768

Size:400ul

Western blot analysis of MOB4A Antibody (C-term) (Cat.#OAAB16768) in Ramos cell line lysates (35ug/lane). MOB4A (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) (Cat.#OAAB16768), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term)(Cat#OAAB16768).NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).MOB4A immunoreactivity is localized to Cytoplasm significantly.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: MOB4A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L
  Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:10~50

Target Info

• Gene Symbol: MOB1B
• Alias Symbols: MATS2, MOB4A, MOBKL1A
• NCBI Gene Id: 92597
• Protein Name: MOB kinase activator 1B
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q7L9L4
• Protein Accession #: NP_775739.1
• Molecular Weight: 25091 Da