Catalog No: OKEH00522
Size:96 Wells
Price: $500.00
SKU
OKEH00522
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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MMP9 ELISA Kit (Rat) : 96 Wells (OKEH00522)

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00522
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MMP9 ELISA Kit (Rat) (OKEH00522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MMP9 captured in well.
ELISA Kit Range0.78 - 50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-MMP9 Microplate96 Wells (12 x 8 Well strips)
MMP9 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MMP91 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.433 ng/mL
SpecificityNatural and recombinant Rat Matrix metalloproteinase-9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP9
Gene Full Namematrix metallopeptidase 9
Alias Symbols92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9
NCBI Gene Id81687
Protein NameMatrix metalloproteinase-9
Description of TargetCould play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.
Swissprot IdP50282
Protein Accession #NP_112317.1
Nucleotide Accession #NM_031055.1
Protein Size (# AA)708
Molecular Weight79 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express MMP9.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express MMP9.
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