MMP9 ELISA Kit (Rat) : 96 Wells (OKEH00522)

Catalog No: OKEH00522

Size:96 Wells

Rat MMP9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MMP9 ELISA Kit (Rat) (OKEH00522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MMP9 captured in well.
• ELISA Kit Range: 0.78 - 50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.2% (n = 20)
  Mean Inter-assay CV%: < 7.4% (n = 20)
• ELISA Kit Component:
  • Anti-MMP9 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP9 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MMP9: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.433 ng/mL
• Specificity: Natural and recombinant Rat Matrix metalloproteinase-9
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP9
• Gene Full Name: matrix metallopeptidase 9
• Alias Symbols: 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9
• NCBI Gene Id: 81687
• Protein Name: Matrix metalloproteinase-9
• Description of Target: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.
• Uniprot ID: P50282
• Protein Accession #: NP_112317.1
• Nucleotide Accession #: NM_031055.1
• Protein Size (# AA): 708
• Molecular Weight: 79 kDa