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MMP9 ELISA Kit (Rat) : 96 Wells (OKEH00522)

96 Wells
$449.00
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Predicted Species Reactivity:
Rat
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
MMP9
Official Gene Full Name:
matrix metallopeptidase 9
Alias Symbols:
92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Matrix metalloproteinase-9, MMP-9
NCBI Gene Id:
81687
Protein Name:
Matrix metalloproteinase-9
Description of Target:
Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.
Swissprot Id:
P50282
Protein Accession #:
NP_112317.1
Nucleotide Accession #:
NM_031055.1
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.433 ng/mL
Kit Range:
0.78 - 50ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology MMP9 ELISA Kit (Rat) (OKEH00522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MMP9 captured in well.
Kit Component:
ComponentAmount
Anti-MMP9 Microplate96 Wells (12 x 8 Well strips)
MMP9 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated MMP91 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MMP9.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MMP9.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Rat Matrix metalloproteinase-9
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
79 kDa
Protein Size (# AA):
708

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143/05/2019 16:34
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Senior Research Technician

Used this kit to test MMP9 levels in rat sciatic nerve damaged versus undamaged. Kit worked perfectly and was very easy to use. Our samples were tissue lysates and we used 10 ug/ml total protein concentration, which seemed to be the perfect amount for the dynamic range of the kit. This was also the least expensive kit that could use tissue lysate for samples. Ordering more after doing our first pilot experiment!

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