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Mmp9 ELISA Kit (Mouse) (OKEH04525)

Catalog#: OKEH04525
Domestic: within 1 week delivery International: 1 week
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~3 Hours
Kit Principle Aviva Systems Biology Mmp9 ELISA Kit (Mouse) (OKEH04525) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mmp9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Mmp9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mmp9 captured in well.
Kit Range 0.156 - 10ng/mL
Kit Reproducibility Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Kit Component
Anti-Mmp9 Microplate96 Wells (12 x 8 Well Strips)
Mmp9 Lyophilized Standard2
Sample Diluent1 x 20 mL
Mmp9 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity 0.086 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Matrix metalloproteinase-9
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol MMP9
Official Gene Full Name Matrix metallopeptidase 9
Alias Symbols 92 kDa gelatinase, 92 kDa type IV collagenase, AW743869, B/MMP9, Clg4b, gelatinase B, Gelatinase B, GELB, Gel B, Matrix metalloproteinase-9, MMP-9, pro-MMP-9
NCBI Gene Id 17395
Protein Name Matrix metalloproteinase-9
Description of Target This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis.
Swissprot Id P41245
Protein Accession # NP_038627.1
Nucleotide Accession # NM_013599.4
Protein Size (# AA) 730
Molecular Weight 81 KDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express MMP9.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express MMP9.
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