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MMP9 ELISA Kit (Human) (OKDD00397)

96 Wells
$489.00
In Stock
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Predicted Species Reactivity:
Human
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology MMP9 ELISA Kit (Human) (OKDD00397) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP9 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP9 captured in well.
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP9 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP9 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Kit Component:
ComponentAmount
Anti-MMP9 Microplate96 Wells (12 x 8 Well Strips)
MMP9 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X MMP9 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage:
Store as indicated in product manual.
Sample Type:
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates
Sensitivity:
< 0.063 ng/mL
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay has high sensitivity and excellent specificity for detection of MMP9. No significant cross-reactivity or interference between MMP9 and analogues was observed.
Assay Info:
Quantitative Sandwich ELISA
Gene Symbol:
MMP9
Official Gene Full Name:
matrix metallopeptidase 9
Alias Symbols:
GELB, CLG4B, MMP-9, MANDP2
NCBI Gene Id:
4318
Protein Name:
matrix metalloproteinase-9
Description of Target:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Swissprot Id:
P14780
Protein Accession #:
NP_004985.2
Nucleotide Accession #:
NM_004994.2
Protein Size (# AA):
707
Molecular Weight:
78 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MMP9.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MMP9.

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