MMP9 ELISA Kit (Human) : 96 Wells (OKAA00024-96W)

Catalog No: OKAA00024-96W

Size:96 Wells

Standard curve of MMP-9 protein using OKAA00024_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human MMP-9 (8x12)
  2. 2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human MMP-9 monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 31 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human MMP-9. The factors listed below were prepared at 200ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-10, MMP-12,
  Recombinant mouse: MMP-2, MMP-3, MMP-9,
  
• Publications:

  Hepatoprotective and anti-tumor effects of targeting MMP-9 in hepatocellular carcinoma and its relation to vascular invasion Markers. Clinical & Experimental Metastasis. 32, 479-493 (2015). 25999065

  More...

• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP9
• Gene Full Name: matrix metallopeptidase 9
• Alias Symbols: GELB, CLG4B, MMP-9, MANDP2
• NCBI Gene Id: 4318
• Protein Name: Matrix metalloproteinase-9
• Description of Target: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
• Uniprot ID: P14780
• Protein Accession #: NP_004985.2
• Nucleotide Accession #: NM_004994.2