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MMP9 ELISA Kit (Human) : 48 Wells (OKAA00024-48W)

  • Catalog#: OKAA00024-48W
  • 2 weeks
48 Wells
$359.00
In Stock
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Application:
ELISA
Gene Symbol:
MMP9
Official Gene Full Name:
matrix metallopeptidase 9
NCBI Gene Id:
4318
Alias Symbols:
92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, MANDP2, Matrix metalloproteinase-9, MMP-9
Description of Target:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Protein Name:
Matrix metalloproteinase-9
Swissprot Id:
P14780
Protein Accession #:
NP_004985.2
Nucleotide Accession #:
NM_004994.2
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
31 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human MMP-9 antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express MMP9.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express MMP9.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay recognizes both natural and recombinant human MMP-9. The factors listed below were prepared at 200ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-10, MMP-12,
Recombinant mouse: MMP-2, MMP-3, MMP-9,
Assay Info:
Assay Method: Quantitative Sandwich ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human MMP-9 (8x12)
2. 2 vials human MMP-9 Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human MMP-9 monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films

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