MMP9 Antibody : Biotin (OASE00244-Biotin)

Catalog No: OASE00244-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S51-82
• Isotype: IgG2a
• Host: Mouse
• Conjugation: Biotin
• Application: IHC, WB, IP
• Additional Information: Background Info: Detecs ~92kDa and ~82kDa (pro and active forms)
  Scientific Background: MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).
  Certificate of Analysis: 1ug/mL was sufficient for detection of MMP9 in 20ug of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 1-708 (full length) of rat MMP9
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Hirose Y., et al. (2008) Am J Hum Genet. 82(5): 1122-1129. 2. Coussens L.M., et al. (2002) Science 295: 2387-2391.
  3. Sternlicht M.D., et al. (1999) Cell 98: 137-146.
  4. Vu T.H., et al. (1998) Cell 93: 411-422.
  5. Nagase H., et al. (1990) Biochemistry 29: 5783-5789.
  

Target Info

• Gene Symbol: Mmp9
• Gene Full Name: matrix metallopeptidase 9
• NCBI Gene Id: 81687
• Uniprot ID: P50282
• Protein Accession #: NP_112317.1