MMP7 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03794)

Catalog No: OKCD03794

Size:96WELLS

Mouse MMP7 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-101%	93-101%	95-105%	85-104%
  Heparin Plasma (n=5)	96-103%	99-105%	80-94%	98-105%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 7 (MMP7) level in the sample or standard.
• ELISA Kit Range: 5.49-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-104	101
  Heparin Plasma (n=5)	87-101	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 7 (MMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Mmp7 Microplate: 96 Wells (12 x 8 Well strips)
  • Mmp7 Lyophilized Standard: 2
  • 100X Mmp7 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.0625 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Mmp7
• Alias Symbols: Matrin;Matrix metalloproteinase-7;Pump-1 protease;Uterine metalloproteinase.
• NCBI Gene Id: 17393
• Protein Name: Matrilysin
• Description of Target: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase (By similarity).
• Uniprot ID: Q10738
• Protein Accession #: NP_034940
• Nucleotide Accession #: NM_010810